| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 |
11권 2호, p.2028 |
| 발표분야 |
생물화공 |
| 제목 |
Parallel Analysis of Antimicrobial Activities in Microbial Community Using Single-Strand Conformation Polymorphism |
| 초록 |
Conventional antimicrobial activity analyses such as broth dilution method are demanding processes for new antimicrobial agent discovery and sensitive diagnosis on the infectious diseases. In this study, we developed a new antimicrobial activity analysis system based on single-strand conformation polymorphism (SSCP) combined with 16S rRNA gene specific PCR. By this method, population change of microbial community in response to the specific antimicrobial agents can be quantified with high sensitivity and accuracy from small amount of sample. Using a mixture of microorganisms containing E. coli, C. glutamicum, and A. calcoaceticus, it was found that the peak areas in 16S rRNA gene specific PCR-SSCP could quantify cell concentration. Subsequently, a comparison study with the broth dilution method using kanamycin, spectinomycin, and streptomycin showed that this method can be used for antimicrobial activity analysis. Results also demonstrated its high potential in the area of clinical diagnosis. |
| 저자 |
정주희1, 김 진2, 박영섭3, 남명희1, 오민규2, 김찬화2, 정규열3
|
| 소속 |
1한국기초과학지원(연), 2고려대, 3포항공과대 |
| 키워드 |
antimicrobial activity analysis; antimicrobial agent; single-strand conformation polymorphism; 16S rRNA gene; microbial community |
| E-Mail |
|
| 원문파일 |
초록 보기 |
