| 초록 |
Titanium and titanium alloy are widely used in medicine because of their biocompatibility, nontoxicity, and good mechanical properties. To add more biofunctionalities, metal surface have been biologically modified using electrodeposition, photoreactivity, or adhesive peptide sequences. However, the methods for surface modification of metal with biological molecules are limited. Conjugating protein to the metal can provide a source of signals to continuously, stably, and efficiently stimulate cells to reconstitute damaged tissues during long-term regeneration. Here, gelatin was modified with phosphate groups as a biological approach to enhancement of the cell adhesion ability of titanium surfaces. The phosphorylation enhanced the binding affinity of gelatin to the titanium surface and the surface modified titanium enhanced attachment of MC-3T3L1 osteoblast cells. Attached cell number on phosphorylated gelatin treated titanium surface was twice as many as non-treated one. |
