| 학회 | 한국화학공학회 |
| 학술대회 | 2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 | 24권 1호, p.371 |
| 발표분야 | 생물화공 |
| 제목 | Comparison of graphite oxide and heme mediator for lactose biosensor using cellobiose dehydrogenase |
| 초록 | Cellobiose dehydrogenases (CDH) structure consists of two distinct domains, dehyrogenase domain and cytrochrome domain, which contains catalytically active flavin adenine dinucleotide (FAD) and heme b, respectively. Based on the enzyme structure, CDH has been repoted in electrochemical applications such as enzymatic biofuel cells and biosensors. In this study, heterologous expression of the cdh from Phnerochate chrysosporium KCCM 60256 in Pichia pastoris X-33 was achieved for efficient production of a recombinanted enzyme (PcCDH). To establish an lactose biosensor system, graphite oxide and heme were selected for mediator composition. The biosensor showed efficient electron transfer and exhibited an extraordinary high current density in a 100 mM lactose solution at pH 4.5. The biosensor detection represented high sensitivity in a large linear range from 1 to 150 mM of lactose solution. The successful demonstration of this study suggests the potential development of lactose biosensor based on the application of PcCDH. |
| 저자 | 최한석1, 김동섭1, 이주훈1, 이수권1, 김형렬1, 박철환2, 유하영3, 김승욱1 |
| 소속 | 1고려대, 2광운대, 3상명대 |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
