| 초록 |
An osteoinductive implant needs to be carefully controlled their composition and surface roughness in order to enhance a new bone formation and biomechanical stability. Bone healing is also improved by the administration of osteogenic factors, such as bone morphogenetic protein-2 (BMP-2). In this study, we hypothesize that the application of BMP-2/CSA nanoparticles can promote the osteointegration of hydroxyapatite (HA)-coated titanium (Ti) implants with the surrounding bone. Human recombinant BMP-2 was incorporated onto the apatite-coated surface with the supersaturated solution of calcium phosphate (SCP). To identify the complexation of BMP-2 and CSA, the nanoparticle was characterized by using SEM, circular dichroism (CD), and zeta potential. The morphology of fabricated HA-coated titanium was determined using SEM, XRD, and EDS. The efficacy and bioactivity of BMP-2 nanoparticles were also evaluated using ELISA kit and W-20 clone 17 cell. |
