| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1574 |
| 발표분야 |
생물화공 |
| 제목 |
Generation of chimeric HBc proteins carrying multiple viral epitopes in E.coli |
| 초록 |
In present study, chimeric virus like particles (VLPs) displaying multiple-epitope of foreign virus were developed in Escherichia coli (E. coli) as vaccine candidate. Two recombinant plasmids encoding HBc::E1::E2 and HBc::E3::A were co-expressed in E. coli, and led to expression of self-assembled chimeric VLPs that are morphologically and physically similar to the wild-type ones. To determine the immunogenicity of these chimeric VLPs, 12 mice were immunized with these particles using Al(OH)3 . As a result, we found that mice immunized with chimeric VLPs were capable of inducing foreign virus specific antibody. This study suggests the potential usefulness of E. coli-derived HBc-VLPs as a carrier for immunogenic presentation and delivery of foreign epitopes and the principal possibility of using HBc-VLPs for creation of a complex vaccine. |
| 저자 |
안금영, 이지원, 송종암, 김경림, 김현진, 유리나
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| 소속 |
고려대 |
| 키워드 |
vaccine; chimeric VLPs; E coli
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| E-Mail |
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| 원문파일 |
초록 보기 |
