| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.659 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Neuronal differentiation of adult bone marrow stromal cells using SIS sponges. |
| 초록 | Bone marrow stromal cells (MSCs) are multipotent stem cells that can be differentiated into mesenchymal cell types such as chondrocytes, osteoblasts, adipocytes, and muscle cells by controlling cytokines in culture medium1,2. Small Intestinal submucosa(SIS) is an acellular biomaterials and consists of naturally occurring ECM that has been shown to be rich in components which support regeneration of tissue such as fibronectin, glycosaminoglycans including heparin, several collagens including types Ⅰ, Ⅲ, Ⅳ, Ⅴ, and Ⅵ, and growth factors such as b-FGF, TGF-β, and VEGF. So, it is to be important for tissue regeneration3,4. In this study, adult rat bone marrow stromal cells can be induced to overcome their mesenchymal commitment and differentiate into neurons in SIS sponge, potentially their usefulness in the treatment of neuronal disease. In vitro, MSCs were seeded in SIS sponges for 14 days. The neurogenesis of MSCs culture was assessed by histology, RT-PCR, SEM, and MTT assay. In histological analysis, we confirmed that neurogenic differentiated rat MSCs expressed NSE and NF. Acknowledgement : This work was supported by KMOCIE (N11-A08-1402-05-1-3). References 1. H. B. Lee, G. Khang, and S. J. Lee, Chap. 41, History and commercialization trend of tissue engineering and organization each countires ; In Tissue Engineering : Concepts and Application, 2nd Eds., edited by J. J. Yoo and I. Lee, pp 751 (2002). 2. A. M. Mackay, S. C. Beck, M. F. Pittenger, and C. O. Chichester, Tissue Eng., 4, 415 (1998). 3. Kristina Lindberg and Stephen F. Badylak, “Porcine small intestinal submucosa (SIS): a bioscaffold supporting in vitro primary human epidermal cell diferentiation and synthesis of basement membrane proteins”, Burns, 27, 254-266 (2001). 4. Stephen F. Badylak, “The extracellular matrix as a scaffold for tissue reconstruction”, seminars in CELL ＆ DEVELOPMENTAL BIOLOGY, 13, 277-383 (2002). |
| 저자 | 김은정1, 송지희1, 신혜원1, 이혜경2, 이일우3, 김문석4, 강길선1, 이해방4 |
| 소속 | 1전북대, 2가톨릭의대 대전성모병원 임상병리과, 3가톨릭의대 대전성모병원 신경외과, 4한국화학(연) |
| 키워드 | MSCs; SIS sponge; neurogenesis |
