| 초록 |
Many studies have revealed that 3,4-dihydroxyphenylalanine (dopa) is a key molecule in underwater adhesion, and underwater adhesion has been well characterized concerning dopa. In sequence, many biomaterials have been suggested based on dopa derived from marine organisms. However, adhesives of marine organisms are made with not only dopa but with other components like lysine. Although comprehension about other components is inevitable to fully mimic their underwater adhesion ability, little is known about them. In this study, we focused on the lysine which is also major components (~15%, dopa is ~20%) of fp-3F, surface adhesion protein of Myilus californianus. We designed peptides with different spacing between dopa and lysine. Nanoscale surface adhesion was measured using surface forces apparatus (SFA) and NMR analysis gave proof about how lysine affect dopa in underwater adhesion. In last, we redesigned actual surface protein from Mytilus californianus and confirmed the actual adhesion enhancement. This study is expected to give insight to every bio-materials that mimics dopa moiety from marine organisms suggesting materials can have higher underwater adhesion ability. |
