| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1754 |
| 발표분야 |
생물화공 |
| 제목 |
Development of PPAR γ2 Aptamer for Regulation of Adipocyte Differentiation |
| 초록 |
Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor, which upon activation increase the growth and differentiation of adipocytes. Adipocytes are the major cellular component in fat tissue and excessive growth, differentiation and hypertrophy of adipocytes are fundamental processes of obesity. The differentiation of preadipocytes into adipocytes involves exposure of a confluent, quiescent population of cells to a variety of effectors that activate a cascade of transcription factors. Here, we prove that inhibiting the differentiation of mouse 3T3-L1 cells into adipocytes; regulate to inhibit PPARγ2. Aptamers are not only useful in their own right, but as escorts for therapeutic or diagnostic reagents. In the present study, aptamer aginst PPARγ2 was selected by SELEX. Aptamers obtained after 10 rounds of selection demonstrated high affinity and specific binding with PPAR γ2 using real-time PCR and SPR system. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2016R1D1A1B03934258) |
| 저자 |
신우리1, 조희영1, Simranjeet Singh Sekhon1, 안지영1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
