| 초록 |
A novel heparin immobilization to small intestinal submucosa(SIS) has been developed. The heparinization involves pretreatment of SIS using glutaraldehyde(GA) and followed by grafting heparin to SIS by the reaction of residual aldehyde and amine group of heparin. SIS was modified by direct coupling of heparin and their blood compatibility evaluated in-vitro. Heparinized SIS was characterized by measuring shrinkage temperature, mechanical properties, digestion resistance to collagenase enzyme, in-vitro activated partial thromboplastin time (APTT) assay, protein adsorption and platelet adhesion and in-vivo calcification. Thermal and mechanical properties showed that the durability of heparin-treated tissue increased as compared with fresh tissue and GA-treated tissue. Resistance to collagenase digestion revealed that heparin-treated tissue has greater resistance to enzyme digestion than did fresh tissue and GA-treated tissue. In-vitro protein adsorption and platelet adhesion study revealed that the lower amounts of protein and platelet were adsorbed and adhered on heparin treated tissue than fresh tissue. In-vivo calcification study demonstrated much less calcium deposition on heparin treated SIS than GA treated one. Obtained results attest to the usefulness of heparin treated SIS for cardiovascular bioprostheses, especially blood vessel application. |
