초록 |
Acidithobacillus ferrooxidans is an chemolithoautotrophic bacteria that generates its energy and reducing power for CO2 fixation from the oxidation of iron- and sulfur-containing compounds. Because of these property, A. ferrooxidans is considered the potential biocatalyst for conversion of CO2 into biochemicals and widely used in bioleaching of metals. During the ferrous iron oxidation by the A. ferrooxidans, the basic ferric sulfates called jarosite tends to be formed and negatively affects process performance including the decrease of ferric iron production, which is used as an oxidizing agent of metals in bioleaching process. Thus, in this study, the culture medium of A. ferrooxidans was statistically optimized to minimize the jarosite formation by using response surface methodology (RSM). As a result of RSM using four independent variables, which are initial pH and initial concentrations of KCl, MgSO4·7H2O and (NH4)2HPO4, it was predicted that jarosite formation would be minimized when the values of initial pH and concentrations of KCl, MgSO4·7H2O and (NH4)2HPO4 were 1.7, 0.05g/L, 0.1g/L and 3.3g/L, respectively. |