| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 | 15권 2호, p.1632 |
| 발표분야 | 생물화공 |
| 제목 | Comparison of α-Amylase activities by immobilizationusing TEOS in the sol-gel process |
| 초록 | As the environmental problems have been increasing nowadays, a biological catalyst which is a renewable resource with little pollutants has been important since it could be applicable to various areas of chemical Engineering. A bio-catalyst loses its activity generally due to heat, strong acid, strong base, organic solvents, etc.; its activity is different depending on the structure of protein constituting the bio-catalyst. Therefore, it would have many advantages by immobilizing the enzyme in appropriate ways. In this study, the activities of free and immobilized enzyme were compared. First, the activity of free α-Amylase was measured at various temperatures. Then, the pore size distribution of TEOS depending on the amount of phosphate buffer was observed by BET. The α-Amylase was immobilized using TEOS in sol-gel process andthe fixed amount of α-Amylase was calculated by absorbance of UV-vis. Finally free and immobilized α-Amylase were compared. |
| 저자 | 김성례1, 정광덕2, 주오심2, 박영권1 |
| 소속 | 1서울시립대, 2한국과학기술(연) |
| 키워드 | Enzyme; Immobilization |
| 원문파일 | 초록 보기 |
