초록 |
Organic Dyes are commonly used to stain cells, proteins or materials or used alone as a probe in a variety of analytical methods. Their photoluminescence(PL) signal plays an important role in the analysis such as imaging, quantitative assay. However, its emission depending on temperature are still behind the veil. Organic dye molecules can undergo unexpected photophysical properties at low temperatures. In this research, It has been observed that the PL properties change considerably at low temperatures below 248 K, unlike at room temperature. We examined several dyes at ultra-low temperature and found that some have enhanced fluorescence, quenched fluorescence, or have dual fluorescence and phosphorescence with different colors. In consequence, we have confirmed that the organic dyes are particularly sensitive to the molecular environment such as phase transition or ice phase at cryogenic temperature, resulting in significant changes in their optical properties. |