| 초록 |
Methodologies to enhance the activity and longevity of enzymes have occupied the significant positions in the enzyme-based biotechnologies. Enzyme immobilization in the solid support has been the most popular methods to fulfil the two key requirements. However, a few reports employed bicontinuous structures for mass transport and nanoscale space for efficient enzymatic functions simultaneously. Recently, we reported caging lipase in nanoporous covalent polymer prepared from the polymer/poly-urea network mixtures via organic sol-gel(OSG) method. The nanoporous membrane could be employed as a trap for enzyme immobilization, with improved lifespan compared to free enzyme. To broaden the scope of enzymes and examine the effects of confinement on the kinetic activity of enzymes, we investigated batch reactions of other categories of enzyme. The result shows the general applicability of caging in nanoporous framework to promote the enzymatic activity with prolonged lifespan. |
