| 초록 |
A variety of methods to increase the sensitivity in polydiacetylene systems were reported in previous papers. Recently, it was published that smaller the vesicles are, the more sensitive is in detecting Con A when polydiacetylene vesicles incorporated with glycolipids were used. In this study, we focused on the effect of sensitivity enhancement of polydiacetylene vesicles in detection α-cyclodextrin according to vesicle size control by filtration with various pore sizes. Vesicles were reacted with different concentrations of α-cyclodextrins, we observed that smaller vesicles induced more colorimetric transition and shortened detection time compared to larger vesicles. These results show that vesicle size is the main factor for enhancing the sensitivity of detection limitation. The effect of vesicle size in detecting biomolecular recognition (DNA-DNA hybridization, protein-protein interaction, antibody-antigen interaction, etc.) is currently under investigation in our group. |
