| 초록 |
Phenylpropanoids are derived from aromatic amino acids and medicinally important plant natural products. In this study, we report the bioproduction of two representative phenylpropanoids, coniferyl alcohol (CA) and dihydroquercetin (DHQ) by engineered Escherichia coli. First, by employing hpaBC , OMT1, 4CL4 and CCR1, CA producing strain produced 187.7 mg/L of CAs. Next, 239.4 mg/L of DHQ was produced by introducing F3H, TT7 and CPR. Signal peptide of cytochrome P450 TT7 was manipulated for higher production. In addition, to produce flavonolignans from glycerol, ascorbate peroxidase was introduced to produce 0.04 μg/L and 1.29 μg/L of silybin and isosilybin respectively by stepwise culture. Finally, 0.12 μg/L isosilybin was produced directly from glycerol, with a single strain expressing 16 essential genes. The engineering strategies here can be useful in the application of producing pharmaceutically important but complex natural products [This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01550602)" Rural Development Administration, Republic of Korea]. |
