| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Plug-in repressor library for precise control of metabolic flux in Escherichia coli |
| 초록 |
Enhanced production of value-added chemicals requires precise flux control between growth-essential and production pathways. Although advances in synthetic biology have facilitated exploitation of various genetic elements, their use requires expensive inducers or time-consuming processes to design and optimize proper regulator components, case-by-case. Therefore, we devised plug-in repressor libraries that expression levels of the repressors were diversified by degenerate 5′ untranslated region sequences. They were applied to improve the production of lycopene from glucose and 3-hydroxypropionic acid from acetate in Escherichia coli. Consequently, we found that the most optimized strains produced 2.59 g/L of 3-HP and 11.66 mg/L of lycopene, 16.5-fold and 2.82-fold increase, respectively, compared to those produced by the parental strains. These results indicate that carbon flux rebalancing using plug-in library is a powerful strategy for efficient production of value-added chemicals. |
| 저자 |
이지연, 차상학, 이지훈, 임현규, 노명현, 강채원, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
Metabolic engineering; Carbon flux rebalancing; 3-Hydroxypropionic acid; Lycopene; UTR Library Designer; Escherichia coli
|
| E-Mail |
|
