| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1215 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Production of aromatic polyester by Escherichia coli using systems metabolic engineering |
| 초록 |
We engineered Escherichia coli strains by integration and overexpression of Clostridium difficile isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase and polyhydroxyalkanoate synthase genes in a D-phenyllactate-producer strain. As a result, poly(52.3 mol% 3-hydroxybutyrate(3HB)-co-47.7 mol% D-phenyllactate) were produced from glucose and sodium 3HB. Various poly(3HB-co-D‐phenyllactate) polymers are produced by expression of Cupriavidus necator phaA and phaB genes. Fed-batch culture of this strain produces 13.9 gL-1 of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). It is the first attempt for one-step fermentative production of aromatic polyesters from renewable resources. [This work was supported by the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) and also by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF2012M1A2A2026556 and NRF-2012M1A2A2026557) from the Ministry of Science and ICT through the National Research Foundation of Korea.] |
| 저자 |
박가은1, 양정은1, 이상엽1, 김원준1, 김형준1, 김범준1, 이혁2, 신지훈2
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| 소속 |
1한국과학기술원, 2한국화학(연) |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
초록 보기 |
