| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Preparation and Characterization of Plasmid DNA-LoadedPLGA/Pluronic F127 Scaffold |
| 초록 |
In recent, the cartilage regeneration using tissue engineering technique and local gene delivery system have been considered as one of the promising therapeutic methods. In this study, we prepared plasmid DNA-loaded PLGA scaffold to enhance the chondrogenic differentiation of mesenchymal stem cells (MSCs). For more effective plasmid DNA release from the scaffold and the following transfection into cells of plasmid DNA, the PLGA scaffold was hydrophilized by Pluronic F127 (can allow faster release behavior of plasmid DNA than hydrophobic one) and the plasmid DNA was complexed with polyethyleneimine-polyethylene glycol (PEI-PEG, non-viral vector). The release behavior of the plasmid DNA/PEI-PEG complex from the hydrophilized PLGA/Pluronic F127 scaffold, transfection efficiency of the plasmid DNA/PEI-PEG complex into MSCs, and the cytotoxicity of the cells were investigated. (This work was supported by a grant from the Korea Ministry for Health, Welfare and Family Affairs). |
| 저자 |
연경선1, 장성환1, 오세행1, 김혜정2, 임군일2, 이진호1
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| 소속 |
1한남대, 2동국대 |
| 키워드 |
Tissue engineering; gene delivery; stem cell
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| E-Mail |
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