| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2979 |
| 발표분야 |
에너지 |
| 제목 |
Effect of buffer on lipase activityduring immobilization process and application of co-immobilization for high activity |
| 초록 |
In our previous work, a method of pretreating lipases was developed and its process was optimized to prevent loss of its activity during covalent immobilization. In this study, the effects of various buffers and ionic strength on immobilization of Rhizops oryzae or Candida rugosa lipases were investigated to enhance the activities of immobilized lipases. Among various buffers, the optimal buffers and ionic strength for the immobilization of R. oryzae and C. rugosa lipases were determined to be the mixture of 0.25 M MOPs and sodium phosphate buffer (pH 6.5). Moreover, activities of immobilized R. oryzae and C. rugosa lipase under their optimal conditions were 3756.11 and 2845.21 U/g matrix, respectively. Moreover, co-immobilization was carried out using optimal buffer conditions and then activity of co-immobilized lipase was 16430.33 U/g matrix. |
| 저자 |
이종호1, 이동환1, 김성봉1, 송윤석1, 강성우1, 임정수2, 박철환3, 태범석4, 김승욱1
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| 소속 |
1고려대, 2삼성전자, 3광운대, 4한경대 |
| 키워드 |
Ebuffer effect; buffer mixture system; pH; ion strength; co-immobilization
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| E-Mail |
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| 원문파일 |
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