| 초록 |
This work aimed to study the bioactivity of oils extracted from a mixture (MX) of citrus seeds (CS) and citrus peels (CP) using supercritical carbon dioxide (SC-CO2) and ethanol as a modifier so that those by-products can be valorized. The extraction conditions were 200 bar and 300 bar at 45 °C, extraction time of 2 h and CO2 flow rate of 27 g/min for neat SC-CO2 and SC-CO2+ethanol. The yield showed to increase significantly (p ＜ 0.05) by increasing pressure. The total phenolic content was determined by Folin–Ciocalteu method and CP oils showed higher value amongst while CS oils showed highest total flavonoid content. The tocopherols and phytosterols were performed by HPLC and α-tocopherol and sitosterol were respectively the main compounds of the extracted oils. The antioxidant activity was determined by DPPH and ABTS assay and the oils extracted by SC-CO2+ethanol at 200 bar showed higher activity with IC50 values of 0.52 and 0.53 mg/ml for CP and MX respectively for DPPH assay. For antimicrobial activity, the MX oils showed higher activity for tested bacteria. Overall the MX oil showed good bio-potentiality which reflects how it could be a tailor-made in many applications. |
