| 학회 |
한국화학공학회 |
| 학술대회 |
2002년 가을 (10/24 ~ 10/26, 서울대학교) |
| 권호 |
8권 2호, p.4073 |
| 발표분야 |
생물화공 |
| 제목 |
종자 단백질의 Foam fractionation에 미치는 pH의 영향 |
| 초록 |
Foam fractionation has the potential to be a cost-effective component of a protein concentration and isolation process. Because it has been thought of primarily as a means to remove water from dilute protein solution mixtures, there has been little application of this process to separate and recover complex protein mixtures such as those found in seed extracts which combine proteins with polysaccharides such as starch and galactomannan. The successful operation of a foam fractionation process depends on the optimization of interaction between solution variables like the pH and the column operating parameters like the gas superficial velocity.In this study, we explore the feasibility of applying foam fractionation to mimosa seed proteins in a water solution. From the measured surface tension-pH profile, strategies are developed to determine the maximum separation of the total protein from the solution to the foam phase, along with the corresponding recovery of those proteins. Eight new proteins (five in significant concentration) were identified in the seeds by their molecular weight. The enrichment ratio in the foam phase for the total protein mixture was found to be relatively insensitive to the feed protein concentration above 100 mg/L at pH 9.0. The enrichment ratio rose from 1.11 at 100 mg/L up to 2.46 at 50 mg/L, while the mass recovery fell from 9.9 % to 3.7 %, respectively. |
| 저자 |
정귀택, 류화원, 조영일, 박돈희, Robert D. Tanner
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| 소속 |
전남대 |
| 키워드 |
Mimosa seed; foam fractionation; protein separation; pH; mimosa seed protein
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| E-Mail |
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| 원문파일 |
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