| 초록 |
Silk is being rediscovered and reconsidered as potentially useful biomaterials in a range of applications. In this study, fabricated silk/PLGA scaffolds using an NP cells were seeded into silk/PLGA 0, 10, 20, 40 and 80wt% scaffolds. After 1, 3 and 7 days of cell seeding, cell proliferation and viability were measured via MTT assay. In addition, morphology of cellular adhesion were observed by scanning electron microscope (SEM). RT-PCR was conducted to confirm mRNA expression of Collagen Ι , Collagen Ⅱ for NP marker. And histological appearance was evaluated by Safranin-O, H&E and MTS. We confirmed that cell viability on silk/PLGA scaffolds of 80wt% was higher than other scaffolds. Also, from RT-PCR and histology staining results, especially, silk/PLGA scaffolds of 80wt% showed the better of that the gene characteristic of NP cells. This research was supported by WCU (R31-20029) and MBC (0405-B001-0204-0006). |
