| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 |
15권 2호, p.1570 |
| 발표분야 |
생물화공 |
| 제목 |
Design ofreactive DNA oligomerfor efficienct immobilizaiton of DNAprobe on the surface |
| 초록 |
To develop reactive DNA oligomer, wefocused on use ofoxanine (Oxa),one of mutagenic lesions, whichis produced fromnitrosative deaminaiton of guanine. SinceOxa hasa kind of activated carboxyl group (O-acylisourea formation) in the base ring,Oxashowsamino group-directed reactivity, whichisusefulforlinkage of DNA probe on the surface.We set upthe synthesis procedurefor chemical preparation of Oxa-containing DNA oligomer (Oxa-DNA)and fabricated DNA microarray by ink-jet spotting of this Oxa-DNA probe on NH2-functionalized glass-slide.Reactive DNA oligomerand its immobilized system willbe usefulfordevelopingadvanced nano/biotech systems. |
| 저자 |
백승필1, 장의경1, 조찬희1, 정현호1, Keisuke Makino2
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| 소속 |
1고려대, 2Kyoto Univ. |
| 키워드 |
reactive DNA oligomer; oxanine;
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| E-Mail |
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| 원문파일 |
초록 보기 |
