| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 봄 (04/11 ~ 04/12, 연세대학교) |
| 권호 | 28권 1호, p.254 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Evaluation of antitumor activity for self-microemulsified BCNU-loaded PLGA wafer against 9L gliosarcoma cells in in vitro |
| 초록 | Interstitial therapy using biodegradable polymeric device loaded with anticancer agent can deliver the drug to the tumor site in high concentration and hence increase therapeutic efficacy. 1,3-bis(2-chloroethyl)-1-nitrosourea(BCNU) is the most commonly used chemotherapeutic agent for brain tumors. But it is unstable to heat and light, and also has a half life of 20min in vitro and less than 15min in vivo by hydrolysis. Self-microemulsion system was developed for enhancement of BCNU half-life from 45min to 130min in vitro. The self-microemulsified BCNU was incorporated into PLGA wafer as a new polymeric implant. Harvested cells were plated in 6-well flat-bottomed plates (2X104 cells/ml). After 24h, free BCNU-loaded PLGA disk or self-microemulsified BCNU-loaded PLGA wafer was placed in contact with the attached cells. After incubation for specific time period, the cytotoxic activity was evaluated by using sulforhodamine B assay (Abs, 520 nm). The results of the experiments were expressed as treatment over control (T/C) values. Cytotoxicity of self-microemulsified BCNU/PLGA wafer against 9L gliosarcoma cells was higher than free BCNU wafer due to the longer half-life of BCNU. Acknowledgment: This work was supported by KMOCIE |
| 저자 | 김선화;이진수;신필경;채강수;정제교;정상영;조선행;강길선;이해방 |
| 소속 | 전북대 |
| 키워드 | BCNU; cytotoxicity; self-microemulsion system; brain tumor; wafer |
