| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Heavy metal adsorption using the Bacillus subtilis spore display system containing his6tag |
| 초록 |
We studied removing heavy metal using Bacillus subtilis spore surface display system. We used cot E protein as an anchoring motif because of its high abundance in coat layer and inserted varying length of histidine tag at the C-terminal end of anchoring motif. We checked the surface expression of histidine tag using flow cytometry with FITC labelled anti-his6-antibody. Histogram of flow cytometry showed higher fluorescence intensity, possibly due to their outer location on Bacillus subtilis spore. We tried heavy metal adsorption with three kind of recombinant spore and DB104(wild type). |
| 저자 |
김준형, 정유라, 최수빈
|
| 소속 |
동아대 |
| 키워드 |
Bacillus subtilis; surface display; cot E
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| E-Mail |
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