| 학회 |
한국재료학회 |
| 학술대회 |
2018년 가을 (11/07 ~ 11/09, 여수 디오션리조트) |
| 권호 |
24권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Anchored protease-activatable polymersome for precise diagnosis of cancer cells |
| 초록 |
Real-time quantitative and qualitative analyses of metastasis-associated proteases are critical for diagnosis and therapeutic treatment of advanced cancers. However, conventional methods based on DNA, peptides, and proteins require sophisticated chemistry and additional processes to expose detection moieties, and they lack elements of temporal control, which limit their applicability. We designed protease-activatable polymersomes (PeptiSomes) for high sensitivity, in situ quantitative analysis of activating membrane-type 1 matrix metalloproteinases (MT1-MMP, MMP14). To do this, we first synthesized an amphiphilic block polymer–peptide and a copolypeptide based on mPEG-b-pLeu and MT1-peptide-b-pLeu, respectively. Amphiphilic self-assembled PeptiSomes in water were capable of disassembling and releasing the encapsulated self-quenched fluorescence dye (calcein) via enzymatic activation by MT1-MMP. Our PeptiSome system may prevent the initiation and progression of cancer metastasis. Furthermore, the PeptiSome approach described here is likely to facilitate the development of rapid protease assay techniques and further extend the role of proteases as metastasis indicators and therapeutic targets. |
| 저자 |
임종우1, 김현욱2, 최지혜1, 이훈재3, 손혜영1, 박근선1, 박채원1, 송대섭2, 허용민1, 함승주1
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| 소속 |
1연세대, 2고려대, 3한국기초과학지원(연) |
| 키워드 |
<P>Polymersome; Cancer; Molecular diagnostics</P>
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| E-Mail |
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