| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Evaluation of various types of scaffold for tissue engineered intervertebral disc using cultured annulus fibrosus |
| 초록 |
We are trying an alternative approach that consists of a functional IVD composed of annulus fibrosus seeded to various scaffolds using tissue-engineering principles. DBP/PLGA, SIS/PLGA, SIS/DBP/PLGA and PLGA scaffolds were prepared by solvent casting/salt leaching. SIS sponge was fabricated by freeze-drying . AF cells were seeded into scaffolds with 2x105 cells. It was evaluated cell proliferation by MTT assay. The implants were removed after 1, 4 ,6 weeks and observed their gross morphology Histological section were confirmed H&E, Safranin-O, Masson's trichrome staining. We observed specific gene expression of disc cell by RT-PCR. Proliferation of AF cells were observed that the PLGA/SIS/DBP scaffold high. Scaffolds maintained theirs morphology. It could confirm aggrecan, type∏ collagen expression by RT-PCR. Acknowledgement This research was supported by the Musculoskeletal Bioorgan Center grant (0405-BO01-0204-0006) from Ministry of Health and Welfare. |
| 저자 |
하현정1, 고연경1, 최방실1, 김순희1, 윤선중1, 박기숙1, 강길선1, 이해방2, 김문석2
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
Annulus fibrosus; PLGA scaffold; cell proliferation; aggrecan
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| E-Mail |
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