| 초록 |
Hyaluronic acid has been widely used as a biomaterial in many tissue engineering applications. Oxidized hyaluronate (OHA) can form hydrogels with glycol chitosan (GC) via Schiff base formation. Interestingly, autonomous healing of OHA/GC hydrogels after damage can be achieved using adipic acid dihydrazide (AAD). In this study, self-healing behavior of OHA/GC/AAD hydrogels as well as their application in 3-D bioprinting was investigated. OHA/GC/AAD hydrogels showed autonomous healing within a few minutes. ATDC5 cells were used as a model chondrocyte, and cell encapsulation caused a slight decrease in the mechanical stiffness of the gels but did not alter the self-healing feature of the gels. Using a 3-D printer, we fabricated hydrogel scaffolds encapsulating ATDC5 cells. Cells were evenly distributed within the gels, and the viability of encapsulated cells was not significantly influenced by the 3-D printing process. This approach may be useful to engineer custom-made cartilage tissue. |
