| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.405 |
| 발표분야 |
생물화공 |
| 제목 |
Pilot-scale Fermentation of Clostridium acetobutylicum for Production of Isopropanol-Butanol-Ethanol Fuel Mixture |
| 초록 |
We aimed at developing an improved fuel alcohol producing Clostridium acetobutylicum strain. A hyper ABE producing BKM19 strain that we previously developed was further engineered to convert acetone into isopropanol. The BKM19 strain was transformed with the plasmid pIPA100 containing the sadh (primary/secondary alcohol dehydrogenase) and hydG (putative electron transfer protein) genes from the Clostridium beijerinckii cloned under the control of the thiolase promoter. The resulting strain produced 27.9 g/L isopropanol-butanol-ethanol (IBE) with negligible amount of acetone. from 97.8 g/L glucose in lab-scale (2 L) batch fermentation. The scalability and stability were evaluated at 200 L pilot-scale fermentation, which showed that the fuel alcohol yield could be improved to 0.37 g/g. [This work was supported by the Advanced Biomass R&D Center of Korea (2011-0028386) through the Global Frontier Research Program of the Ministry of Education, Science and Technology. Further support by GS Caltex and BioFuelChem are appreciated.] |
| 저자 |
천승우1, 장유신1, 이종민1, Julia Lee2, Moon-Ho Eom2, Do Young Seung2, 이상엽1
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| 소속 |
1KAIST, 2GS Caltex R&D Center |
| 키워드 |
ABE fermentation; Pilot; Isopropanol; Clostridium acetobutylicum
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| E-Mail |
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| 원문파일 |
초록 보기 |
