| 초록 |
Adult stem cells (ASCs) are excellent candidates for cell therapy applications but differentiation control and expansion of cultures are often difficult tasks. For human neural stem cells (hNSCs), in vitro models involving AD and toxicology screening have been successfully developed. Here, we will report our efforts involving a naturally-occurring polysaccharide called hyaluronan (found in brains) and how it was designed to serve as a biomimetic soft substrate that recapitulates the mechanical properties of the brain. The biological behavior of human neural stem cells called ReNcell VM was investigated in response to molecular interactions enabled by a cell penetrating peptide (CPP) and a crosslinked hydrogel system. We will specifically report the synthesis of methacrylated HA (MeHA) polymer from an inexpensive cosmetic-grade hyaluronan starting material. Crosslinked MeHA hydrogels were prepared using photo-initiated radical polymerization processes. We assessed cellular adhesion, spreading, proliferation, and stiffness-dependent neuronal differentiation properties of ReNcell VM human neural stem cells (hNSCs) and compared the results to previous reports in the literature. |
