| 초록 |
A variety of tissue engineering approaches are motivated by the clinical need for articular repair. Non-Purify alginate and purify alginate were fabricated by seeded cartilage cell on the sponge. After 1, 5, 7, 10 and 14 day of cell seeding, cell proliferation activity was measured via MTT assay. Morphological observation, histology, biological assay for collagen type I, II and aggrecan, and PCR were performed at each time point 1, 2 and 3 weeks. In result, the cell viability was better in purify alginate sponge. SEM results showed that more attach cell in the purify alginate sponge with lapse of time. DMMB results showed that the highest content of GAG and collagen levels at purify alginate sponge. Compression results indicated that purify alginate sponge was the highest intensity. This study suggested that purify alginate sponge may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage. |
