| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Anti-Senescence Effect of Thermo Responsive Nanofiber Surface(PIPAAm) Versus Trypsinization on Adiopose Tissue Stem Cell. |
| 초록 |
Cell harvest using enzymatic digestion, such as trypsinization, results in degradation of cell surface proteins and trypsin might have negative effect such as accelerating cell aging and damage. In this study, we investigated that thermo responsive cell culture nanofiber(PIPAAm) versus trypsin on ATSCs passage 2 and passage 7 respectively and detected senescence marker, β-gal activity that SA-β-gal is present only in senescence cells and TRAPeze Telomerase Detection assay that detect telomerase. Thermo responsive nanofiber is more effective anti-senescence than trypsinization in ATSCs. |
| 저자 |
오은정1, 김태정1, 임현주1, 정소연1, 권오형2, 정호윤1, 최진현1
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| 소속 |
1경북대, 2금호공과대 |
| 키워드 |
PIPAAm; TRAP
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| E-Mail |
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