| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Effect of DBP in DBP/PLGA scaffold for Articular Cartilage tissue engineering |
| 초록 |
A solvent casting/salt-leaching method for the preparation of DBP/PLGA scaffolds was designed by varying the DBP contents with 0, 10, 20, 40 and 80 wt %. The DBP contents of the DBP/PLGA scaffold influenced the cell viability and extracellular matix secretion. Artiular chondrocytes of Newzealand White Rabbits were in vitro cultured for 3 weeks. To charterize chondrocyte of MTT, GAG assay, RT-PCR, H&E, Safranin-O stanining were evaluated. As DBP contents of these scaffolds become higher, the rate of cell growth and the amount of GAG increase. The DBP/PLGA scaffolds with DBP content from 10 to 20 wt % are better cell proliferation, ECM secrection and the gene expressions of chonrocyte. Chondrocytes prefer the group of PLGA scaffolds with DBP contents from 10 to 20 wt % for better proliferation and ECM production. It is believed that DBP would significantly affect the cell metabolism. This study is supported by WCU(R31-20029) and MBC (0405-BO01-0204-0006). |
| 저자 |
배지연, 김형은, 유현, 이선경, 김온유, 안우영, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
PLGA; DBP; Condrocyte
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| E-Mail |
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