| 학회 |
한국고분자학회 |
| 학술대회 |
2016년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
41권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
In-situ formation of protein-polymer nanoparticles by covalent conjugation |
| 초록 |
We report covalent bioconjugation of N-hydroxysuccinimidyl-end-functionalized polystyrene (NHS-PSt) with primary amino groups of lysine residues in protein to form in situ core-shell polymer-protein hybrid spherical nanostructures. For this, we synthesized N-hydroxysuccinimidyl-end-functionalized polystyrene by atom transfer radical polymerization (ATRP) using N-hydroxysuccinimidyl-2-bromo-2-methylpropionate initiator. The polymer was characterized by gel permeation chromatography and 1H NMR. For bioconjugation, NHS-PSt dissolved in DMF was added slowly to deionized water containing protein at pH 8 at room temperature in a glass vial. Self-assembled protein-polymer hybrid core-shell spherical nanostructures were characterized using DLS and TEM studies. The stability of hybrid nanostructures was also studied over time. |
| 저자 |
이태헌1, 이채연1, 채민수1, 정종화1, aruna kumar mohanty1, 최지은2, 송재광2, Mohammad Abdul Kadir1, 백현종1
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| 소속 |
1부산대, 2한국화학(연) |
| 키워드 |
Atom transfer radical polymerization (ATRP); Bioconjugation; Self-assembly; Nanostructures; Polymer protein conjugation
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| E-Mail |
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