| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 가을 (10/27 ~ 10/29, 광주 김대중컨벤션센터) |
| 권호 |
27권 2호, p.1727 |
| 발표분야 |
생물화공 |
| 제목 |
Repeated Usage of Bacillus subtilis Spore Displayed Tyrosinase for the Biotransformation of Phloretin |
| 초록 |
In this study, we tried the biotransformation of phloretin using Bacillus subtilis spore displayed tyrosinase from Bacillus megaterium. Phloretin is mainly found in the root bark of apple trees and in apples where it acts as a natural antibacterial plant defense metabolite. The cell surface expression of tyrosinase was verified by flow cytometry using FITC labelled anti-His6tag antibody. After 7 hr of incubation at 37°C, we confirmed that phloretin was biotransformed into a new product by HPLC analysis. And It was further analyzed using LC/MSMS and 1H-NMR to be revealed as 3’-hydroxyphloretin. In addition, the spores were washed with Tris-HCl buffer and reused in the reaction. The biotransformation proceeded through 7 recycling reactions without showing any decrease of the enzymatic activity. |
| 저자 |
정유라1, 박찬진2, 김준형3
|
| 소속 |
1동아대, 2Brain Busan 21 Plus Program, 3Graduate School |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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