| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.399 |
| 발표분야 |
생물화공 |
| 제목 |
Isopropanol-Butanol-Ethanol (IBE) Fermentation using Clostridium acetobutylicum and Its Derivative |
| 초록 |
A primary/secondary alcohol dehydrogenase (SADH, encoded by adhI) from Clostridium beijerinckii NRRL B-593 was introduced into C. acetobutylicum ATCC 824 under the control of adc promoter. The resulting strain was able to produce isopropanol with trace amount of acetone.In order to furtherincrease isopropanol and butanol production, a synthetic acetone operon (act operon) consisting of three homologous genes (adc, ctfA, and ctfB) was constructed using the adc promoter. Simultaneous expression of act operon and adhI in C. acetobutylicum ATCC 824 resulted in increased isopropanol production, and the butanol titer was comparable with wild-type in the flask culture. Further increase of total alcohol titer was achieved using C. acetobutylicum PJC4BK, where the butyrate kinase gene is inactivated. [This work was supported by the Advenced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology (2010-0029799). Further supports by GS Caltex, BioFuelChem, and the World Class University Program (R32-2008-000-10142-0) through the National Research Foundation of Korea funded by the MEST are appreciated.] |
| 저자 |
이종민1, 장유신1, 조정희2, 송효학2, 이상엽1, 승도영2
|
| 소속 |
1KAIST, 2GS칼텍스 |
| 키워드 |
Clostridium acetobutylicum; Biobutanol; Isopropanol
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
