| 초록 |
Calcium phosphate cements (CPCs) have been used to replace bone graft materials because of its biocompatibility and other attractive features. Especially, injectability of CPC allows easy handling for operation with minimally invasive surgical techniques. However, current developed CPCs have a limitation because of the absence of interconnected pores structure and slower degradation rate. For this reason, we developed the new system using brushite based CPC incorporated with polyelectrolyte fibers. By loading chitosan-alginate fiber, which is produced by interaction between polycation (chitosan) and polyanion (alginate) in the CPC, mechanical properties of the final CPC increased as well as its biocompatibility. First, we optimized the liquid/solid ratio of cement components and investigated compressive strength, setting time, pH change, injectability of CPC (without fiber) and FCPC (with 0.5%, 1%, 1.5% fiber). Based on the result, optimized CPC composition was selected for in vivo studies. FCPC and CPC both samples were implanted in the rabbit’s femoral head and kept for 1 and 3 months, and then extracted to check bone formation behavior. Micro-CT and histological analysis by hematoxylin and eosin (H&E) and masson’s trichrome staining reveald a superior bone formation behavior on polyelectrolyte fiber loaded CPCs. Based on the results, we were able to confirm that the use of polyelectrolyte fiber in CPC system could not only provide a biomimetic bone structure but also have a synergistic effect for bone regeneration. |
