| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Influence of Alginate/Demineralized Bone Particle Sponges using Chondrocytes on the Articular Cartilage Tissue Engineering |
| 초록 |
Alginate and DBP sponges were fabricated by 10, 20, 40 and 80 wt% alginate/DBP hybrid sponges for seeding cartilage cell. Cell proliferation activity was measured via MTT assay. Biological assay for collagen type I, II and aggrecan, and PCR were performed at each time point 1, 2 and 3 weeks. The cell viability was better in 20 wt% alginate/DBP sponges than other wt% alginate/DBP sponges. Scanning electro microscope (SEM) results showed that more attach cell in the 20 wt% alginate/DBP sponges with the lapse of time. Dimethylmethylene Blue (DMMB) results showed that the high content of Glycosaminoglycan (GAG) and collagen levels at 20 wt% alginate/DBP sponges. This study suggested that alginate/DBP sponge may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage. This research was supported by the Brain Korea 21 PLUS Project, Bio-industry Technology Development Program(KMFAFF, 112007-05-2-SB010). |
| 저자 |
이선의, 국현, 김수민, 김은영, 송정은, 강길선
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| 소속 |
전북대 |
| 키워드 |
Chondrocytes; Demineralized bone particle(DBP); Alginate; Articular cartilage tissue engineering
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| E-Mail |
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