| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
[정밀화학] 정밀화학 기술 - COVID-19 시대 |
| 제목 |
Development of a therapeutic antibody candidate targeting a novel target in colorectal cancer |
| 초록 |
Cetuximab is a human/mouse chimeric monoclonal antibody effective in a limited number of CRC patients because of cetuximab resistance. In this study, through phage display technology, we first isolated a fully human antibody strongly binding to the cetuximab-resistant HCT116 cell surface and identified the target antigen as glucose-regulated protein 94 (GRP94) using proteomic analysis. In vitro functional studies revealed that the GRP94-blocking antibody we developed strongly inhibits the growth of various cetuximab-resistant CRC cell lines. We also demonstrated that GRP94 IgG monotherapy significantly reduces HCT116 cell growth more potently compared to cetuximab, without severe toxicity in vivo. Therefore, cell surface GRP94 might be a potential novel therapeutic target in cetuximab-resistant CRC, and antibody-based targeting of GRP94 might be effective for suppressing GRP94-expressing cetuximab-resistant CRC. |
| 저자 |
이석묵 |
| 소속 |
국민대 |
| 키워드 |
Cetuximab resistance; GRP94; Human antibody; Colorectal cancer
|
| E-Mail |
|
