| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
On-chip analysis of high producing strains for chemical production in microfluidic static droplet array |
| 초록 |
Combinatorial engineering is a powerful way to create microbes for economic production of chemicals. However, current methods place constraints on monitoring of intracellular concentration of diverse target products at a single cell level. Here, we established a high-throughput screening platform for assessment of metabolites based on microfluidic static droplet array (SDA) and artificial riboswitch. In this system, a genetically encoded riboswitch visualizes intracellular concentration of a target molecule in a quantitative manner. Single cells from a library are individually entrapped in SDA to detect and collect highly productive variants. Using this approach, we analyzed intracellular L-tryptophan concentrations of single Escherichia coli cells with fluorescence from an artificial tryptophan riboswitch. A mutant library was screened for improved tryptophan production and superior strains were obtained exhibiting up to 145% productivity in comparison with their parental strain. |
| 저자 |
황재성1, 정규열1, 장성호2, 장성연1, 김성경3
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| 소속 |
1POSTECH, 2인천대, 3CJ |
| 키워드 |
Tryptophan; static droplet array; on-chip; combinatorial engineering
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| E-Mail |
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