| 초록 |
Mitochondria are important subcellular organelles for cell death and energy production. Mitochondrial dysfunction contributes to many human diseases including neurodegenerative disease, cancer and diabetes mellitus. Approaching this organelle would create a powerful means for treating various diseases described above. However, delivering bioactive materials to the cytoplasmic mitochondria is restricted by the presence of the plasma membrane and mitochondrial membrane. To overcome this problem, we introduced a cationic oligo-peptide composed of histidines and arginines (H3R9) to the mitochondria-targeting sequence (MTS) based on saccharomyces cytochrome oxidase IV. Synthesized MTS-H3R9 was efficiently translocate to the cytoplasmic mitochondria in HeLa cells according to confocal laser microscopy and flow cytometry studies. |
