| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.652 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Tissue engineered disc tissue in a various types of porous scaffolds in vivo |
| 초록 | The aim of tissue engineering is to allow the body to repair itself by scaffolds, and biological signals to damaged or diseased tissues and organs. The form of polymer scaffold has to take porous structure that can diffuse well, induce form of blood vessle rapidly.1-3 There is no optimal treatment for the persistent pain associated with intervertebral disc degeneration. Since fixation of the spine has met with limited clinical success, focus has shifted toward replacement using prosthetic artificial intervertebral discs(IVD).4 In this study, we are developing an alternative approach that consists of generating a functional spinal unit in vitro composed of disc cells anchored to various scaffolds using tissue-engineering principles. The scaffolds are PLGA(poly(L-lactide-co-glycolide)), PLGA/SIS(small intestimal submucosa), PLGA/DBP(demineralized bone particle), PLGA/SIS, PLGA/SIS/DBP, SIS sponge, PGA(poly glycolic acid) mesh. Morphology of scaffolds were identified, we implanted scaffolds seeded rabbit disc cells to nude mouse. Dissection was confirmed after 1 and 2 weeks, performed staining; Hematoxylin and eosin, immunohistochemical staining for collagen type I·II, safranin-O staining. We found that formed disc tissue in SIS sponge by staining. The result of this experiment indicated that SIS sponge is the best scaffold among these scaffolds to grow and differentiate disc cells. Acknowledgement : This work was supported by KMOHW (0405-BO01-0204-0006). Figure 1. Morphology of scaffolds by SEM Reference 1. D. J. Mooney, R. Langer, Biomaterials, 17, 115 (1996) 2. J. W. Jang, B. Lee, H. W. Whan, et. al., Polymer(Korea), 28, 382 (2004) 3. H. W. Shin, S. H. Kim, J. W. Jang, et. al., Polymer(Korea), 28, 194 (2004) 4. C. A. Seguin, M. D. Grynpas, Spine, 29, 1299 (2004) |
| 저자 | 박기숙1, 김순희1, 최방실1, 하현정1, 조윤경2, 한창환2, 김문석3, 강길선1 |
| 소속 | 1전북대, 2대전성모병원 정형외과, 3한국화학(연) |
| 키워드 | Artificial intervertebral disc; scaffold; SIS; DBP; BMSC |
