| 초록 |
In this paper, we develope a novel cell docking system that combines with microvalves and microwells. Conventional cell docking device based on micro-well suffers from generation of dead volume and shear stress within micro-wells resulting in low efficiency of cell docking, limitation of nutrient, and low cell viability. The efficiency of cell docking into microwells is varied with several experimental parameters including flow rate, cell concentration, micro-valve pressure, and shape of microstructure. Integration of microvalve on microwells is able to minimize the behavior of dead volume and eddy in microwell as well as shear stress. Especially, the microvalves can change flow dynamics to induce localization of cells in desired locations. We examined the effect of microvalves in the cell docking process by controling the valve pressure, shape, and size. We found the optimal flow rate and valve pressure to obtain high efficiency of cell docking in microwells. Therefore, this microfluidic device could offer a platform for a wide range of applications such as cell based assay and cell biology. |
