| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1771 |
| 발표분야 |
생물화공 |
| 제목 |
Genetic Engineering of YJ003-OTBP2 strain of S. venezuelae for the production of TDP-4-amino-4, 6 dideoxy-glucose |
| 초록 |
Deoxyaminosugars comprise an important class of deoxysugar moieties synthesized by a variety of organisms, including plants, fungi, and bacterial, which are usually formed via transamination reaction. Deoxyaminosugars are indispensable structural components of many biologically active natural products and are essential for many significant cellular processes These features of deoxyaminosugars make studies of their synthesis important in efforts to produce antibiotics with new deoxysugar appendages by reengineering biosynthetic pathways either in vivo or in vitro. Here, pOTBP1 plasmid, based on the integrative plasmid pSET152 derivative containing an ermE* promoter has been constructed and transformed in YJ003 strain of S. venezuelae by PEG mediated protoplast transformation method. Expression vector i.e. pOTBP2 containing 4-aminotrasferase gene (gerB) from S. sps Geri-155 has later transformed in new mutant YJ003-OTBP1 to get YJ003-OTBP2 for the production of TDP-4-amino-4, 6 dideoxy-glucose. |
| 저자 |
이희찬, Binod Babu Pageni, 오태진, 류광경, 송재경
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| 소속 |
선문대 |
| 키워드 |
Deoxyaminosugar; Integrated plasmid; TDP-4-amino-4; 6 dideoxy-glucose
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| E-Mail |
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| 원문파일 |
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