| 초록 |
Bacterial-infected keratitis is a common disease and sometimes induces severe symptoms such as blindness. Nitric oxide (NO), a cell signaling molecule, has been exogenously applied in physiological systems for its pharmaceutical functions including bactericidal and wound healing effects. However, controlled release of NO is an important issue because gas diffusion behavior is difficult to control and NO performs depending on its concentration and time. In this study, we controlled NO release of N-diazeniumdiolate which is a chemical donor spontaneously releasing NO from surface-modified silica NPs with branched polyethylene imine (BPEI). NO donors were stabilized by near amine groups of BPEI by hydrogen bonding for controlled release. BPEI-surface modified NO-releasing NPs (BPEI-NO NPs) presented 3.5 umol/mg of NO release per a day. Based on in vitro non-cytotoxicity and bactericidal effects of NPs, we examined the ocular wound healing efficiency of BPEI-NO NPs in vivo keratitis model. |
