| 초록 |
RNA structures have been employed widely as therapeutics. However, immunostimulatory effect mediated by RNA construction remains a hurdle for clinical translation. To investigate immunocompatibility, RNA microsponges (RNAMs) were self-assembled by rolling circle transcription. RNAMs are composed of single or double stranded RNA strands and rationally designed to have micron-size for efficient uptake by macrophages. RNAMs exhibited outstanding resistance to serum nucleases, but selectively released RNA strands under endolysosomal conditions. Regardless of intrinsic immunogenicity of free RNA strands, RNAMs triggered only basal or negligible expression of pro-inflammatory cytokines from macrophages and dendritic cells. Taken together, RNAMs showed immunocompatibility within the therapeutic dosage, while a systemic release of RNA strands would give RNAMs a potential for immunomodulatory effect. |
