| 초록 |
We developed a novel synthetic approach to produce cell-targetable DNA-RNA hybrid nanovectors. This material was generated by the dual enzyme polymerization, especially rolling circle amplification (RCA) for DNA and rolling circle transcription (RCT) for RNA. DNA strands contains tumor-specific aptamer and RNA strands includes the siRNA precursors. In further steps, DNA-RNA hydrogel embedding the therapeutic particles was also fabricated. In previous studies, there are few reports in regard to RNA hydrogel due to the structural instability. To overcome this limitation, we employed dual polymerization resulting in macroscale RNA-based structure. This system is considered as a smart double-sustained release of RNA particles from hydrogel then siRNA from the particles. As a proof of concept, the hydrogel was applied to green fluorescence protein (GFP) silencing assay. Taken together, DNA-RNA hybrid materials has high potential to capacitate further exploration in gene therapy. |
