| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.469 |
| 발표분야 | 에너지 |
| 제목 | Metabolic Redesign of E. coil for Hydrogen Production by Activation of Pentose Phosphate Pathway |
| 초록 | H2 is widely recognized as a clean and efficient energy resource in the future due to its high energy content per unit weight as well as CO2-neutral by-product. Biological hydrogen production is less energy-intensive and environmentally benign process than other chemical or electrochemical-based processes. Anaerobic fermentation has advantages such as light source-independence and availability of various substrates (i.e. pure biomass or waste products). However, the theoretical hydrogen production yield per 1-mol glucose is limited to maximum 2-4 mol of H2. In this study, we redesigned metabolic pathway of Escherichia coli to maximize the reducing power by activation of pentose phosphate pathway (PPP) for increasing hydrogen production as following strategies. 1) [FeFe]-hydrogenase system of C. acetobutylicum and Ferredoxin/NAD(P)H:Ferredoxin oxidoreductase genes were introduced into E. coli BL21(DE3) for hydrogen production and electron transfer. 2) zwf gene to drive metabolic flux into PPP and glpX gene in glucogenic pathway to complete activating cycle were overexpressed. |
| 저자 | 김영미, 조한샘, 정규열, 박종문 |
| 소속 | POSTECH |
| 키워드 | biological hydrogen production; metabolic engineering; pentose phosphate pathway |
| 원문파일 | 초록 보기 |
