| 초록 |
Histone deacetylase (HDACs) play important roles in regulating various physiological and pathological processes such as gene expression, cell cycle, and tumorigenesis. These enzymes activity can be attractive targets for drug development. In this study, we synthesized a fluorescent probe, based on conformational conversion of peptidyl receptor, by incorporating the Kac residue and the O-NBD moiety. We expected that upon enzymatic removal of the acetyl group in the Kac residue, the released free amine reacted intramolecularly with the O-NBD moiety, resulting in increase the dynamic range of fluorescence intensity. |
