| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-정밀화학 |
| 제목 |
Design and synthesis of fluorescence peptide-based probes with NBD and sNBD fluorophore for detecting biothiols in aqueous buffered solution and live cells. |
| 초록 |
Amino-terminal copper and nickel(ATCUN) binding motif(NH2-XXH) have been used as an ensemble system with Cu(II) (probe-Cu2+) because the binding motif had a potent binding affinity for Cu(II) and Cu(II) fully quenched fluorophore of ATCUN binding motif. We synthesized fluorescent labeled peptides corresponding to ATCUN binding motif using NBD and sNBD fluorophores. The ensemble system with Cu(II) detected important biothiols including H2S. The addition of H2S promptly took Cu2+ from the ensemble in pure buffered aqueous solutions, causing a “turn-on” fluorescent response. The ensemble sensitively detected the low concentration of H2S. In this feature article, we will discuss probes using ensemble system with Cu(II) in a buffered aqueous system and the selectivity of biothiols. |
| 저자 |
류기, 이건형
|
| 소속 |
인하대 |
| 키워드 |
Fluorescence; Sensor; Biothiol; ATCUN binding motif
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| E-Mail |
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